macrophage colony (MedChemExpress)
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MedChemExpress
macrophage colony
Macrophage Colony, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macrophage+colony/M-CSF%2C+Mouse/pmc13471083-120-17-23
Average 98 stars, based on 35 article reviews
Macrophage Colony, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macrophage+colony/M-CSF%2C+Mouse/pmc13471083-120-17-23
Average 98 stars, based on 35 article reviews
macrophage colony - by Bioz Stars,
2026-09
98/100 stars
Images
1) Product Images from "APOE -associated lipid-handling macrophages in hepatocellular carcinoma: ligand–receptor communication and host Apoe -linked myeloid remodeling"
Article Title: APOE -associated lipid-handling macrophages in hepatocellular carcinoma: ligand–receptor communication and host Apoe -linked myeloid remodeling
Journal: Frontiers in Immunology
doi: 10.3389/fimmu.2026.1902143
Figure Legend Snippet: Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, macrophage colony-stimulating factor; BMDMs, bone marrow-derived macrophages.
Techniques Used: Isolation, Staining, Flow Cytometry, Fluorescence, Two Tailed Test, Derivative Assay
Related Articles
Isolation:Article Title: Mitochondrial metabolism restoration via Tramiprosate suppresses mitochondrial ROS-driven foamy macrophage senescence post spinal cord injury Article Snippet: After weighing, the MD was resuspended in sterile PBS (KGL2210, KeyGEN, Nanjing, China) to a final concentration of 100 mg/mL ( ). .. ● To obtain primary BMDMs, bone marrow was isolated from 8-week-old mice and resuspended in dulbecco's modified eagle medium (DMEM; KeyGEN) medium supplemented with 30 ng/mL Article Title: L-Lactate reprograms tumor-associated macrophages to drive pancreatic cancer progression via BCL3 lactylation Article Snippet: To generate macrophage-like phenotypes, THP-1 monocytes were differentiated using 100 ng/mL phorbol 12-myristate 13-acetate (PMA; MedChemExpress, Cat# HY-18739) for 24 hours. .. Primary bone marrow-derived macrophages (BMDMs) were isolated from the femurs and tibias of 6- to 8-week-old C57BL/6J mice and differentiated for 7 days in RPMI-1640 medium containing 20 ng/mL Article Title: Mitochondrial metabolism restoration via Tramiprosate suppresses mitochondrial ROS-driven foamy macrophage senescence post spinal cord injury. Article Snippet: After weighing, the MD was resuspended in sterile PBS (KGL2210, KeyGEN, Nanjing, China) to a final concentration of 100 mg/mL (Figure S1B). .. ● To obtain primary BMDMs, bone marrow was isolated from 8-weekold mice and resuspended in dulbecco's modified eagle medium (DMEM; KeyGEN) medium supplemented with 30 ng/mL Modification:Article Title: Mitochondrial metabolism restoration via Tramiprosate suppresses mitochondrial ROS-driven foamy macrophage senescence post spinal cord injury Article Snippet: After weighing, the MD was resuspended in sterile PBS (KGL2210, KeyGEN, Nanjing, China) to a final concentration of 100 mg/mL ( ). .. ● To obtain primary BMDMs, bone marrow was isolated from 8-week-old mice and resuspended in dulbecco's modified eagle medium (DMEM; KeyGEN) medium supplemented with 30 ng/mL Article Title: Functional characterization and engineering of the sugar donor specificity of a 2″-O-xylosyltransferase from Panax notoginseng for rare saponins biosynthesis. Article Snippet: 2′′-O-xylosylation is a rare glycosylation pattern conferring unique bioactivities to triterpenoid saponins in Panax species.. Here, we identify a novel 2′′-O-xylosyltransferase, PnUGT94X1, from P. notoginseng, which regioselectively catalyzes 2′′-O-xylosylation at the C3-linked sugar of diverse saponins.. PnUGT94X1 exhibits a pronounced preference for UDP-xylose and a broad specificity tolerance for sugar acceptors including protopanaxadiol-, cycloartenol-, and oleanane-type saponins. Article Title: Mitochondrial metabolism restoration via Tramiprosate suppresses mitochondrial ROS-driven foamy macrophage senescence post spinal cord injury. Article Snippet: After weighing, the MD was resuspended in sterile PBS (KGL2210, KeyGEN, Nanjing, China) to a final concentration of 100 mg/mL (Figure S1B). .. ● To obtain primary BMDMs, bone marrow was isolated from 8-weekold mice and resuspended in dulbecco's modified eagle medium (DMEM; KeyGEN) medium supplemented with 30 ng/mL Cell Culture:Article Title: Targeting CXCL16-expressing macrophages with a biomimetic nanocarrier system attenuates cartilage degeneration in osteoarthritis. Article Snippet: Osteoarthritis (OA) is a whole-joint disorder characterized by progressive cartilage degradation and chronic synovial inflammation, in which macrophages play a central role.. Using single-cell RNA sequencing of human and mouse synovial tissues, we identified a pronounced expansion of macrophages during OA progression, with nearly ubiquitous expression of the chemokine CXCL16.. These CXCL16 macrophages exhibited a proinflammatory phenotype and were strongly associated with synovitis. Article Title: Functional characterization and engineering of the sugar donor specificity of a 2″-O-xylosyltransferase from Panax notoginseng for rare saponins biosynthesis. Article Snippet: 2′′-O-xylosylation is a rare glycosylation pattern conferring unique bioactivities to triterpenoid saponins in Panax species.. Here, we identify a novel 2′′-O-xylosyltransferase, PnUGT94X1, from P. notoginseng, which regioselectively catalyzes 2′′-O-xylosylation at the C3-linked sugar of diverse saponins.. PnUGT94X1 exhibits a pronounced preference for UDP-xylose and a broad specificity tolerance for sugar acceptors including protopanaxadiol-, cycloartenol-, and oleanane-type saponins. |